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Figure Lengend Snippet: Activation, redistribution, and release of ALU elements in ETO-treated cells: (A) The result of qRT-PCR for ALU repeats in the PA1-ETO experiment showing ∼3-fold increase of ALU transcription by day 5 post-treatment; (B) NT control – the relatively even distribution of ALU in cell nuclei; (C) clustering of fused ALU elements around nucleoli after ETO treatment; (D, E) colocalization of ALU with 45S rDNA in the nuclear (arrow) and perinuclear (arrowhead) NoA after ETO-treatment as revealed by double FISH; (F) chaotic release of large fused ALU drops from the cell nucleus after ETO treatment. Bars = 10 µm.

Article Snippet: ALU fragments were labeled with Cy3 (Amersham, red fluorescence) and rDNA fragments with ATTO 390 (Jena Bioscience, blue fluorescence) according to a standard oligo-labeling protocol.

Techniques: Activation Assay, Quantitative RT-PCR